Abstracto

In Vitro Cytotoxic, In Vitro and In Vivo Antidiabetic Activity of Roylea Cineria

R. P. Bahuguna, J. S. Jangwan, R. Singh and U. P. Bhatt

Roylea cineria belong to family Lamiaceae is an evergreen, erect or scandent aromatic shrub found at 2000-5000 ft high. It is commonly known as Karui. In vitro cytotoxic activity of ethanol extract was carried out against different cell lines SK-Mel 41, U-87 MG, Hela, MDA-MBA-231 cell line and LC50 value was determined was found to be 131.8 μg/mL, 275.4 μg/mL, 302.0 μg/mL, respectively. In vitro anti-diabetic potential of EtOH extract (UB-1), petroleum ether extract (UB-2), ethyl acetate extract (UB-3), MeOH extract (UB-4) was tested, which showed maximum of 65.7%, 39.0%, 42.9% and 61.3% PTP-1B% inhibition, respectively at 100 μM concentration. IC50 value for UB-1 and UB-4 was found to be 7.5 μm and 9.1 μM, respectively. In vivo anti-diabetic potential of EtOH extract (UB-1), petroleum ether extract (UB-2), ethyl acetate (UB-3), MeOH extract (UB-4) were tested for lowering the blood glucose level of streptozotocin-induced diabetic rats. UB-2, UB-3 and UB-4, which showed decline in blood glucose level to the tune of 23.4% (p < 0.01), 18.2% (p < 0.01) and 20.7% (p < 0.01) during 0-24 hr, respectively at 100 mg/Kg oral dose