Abstracto

Influence of sucrose on morphogenetic changes of four rare and endangered ferns of western ghats, South India

M.Johnson, V.S.Manickam

The present study was intended to study the effect of sucrose on spore germination, gametophyte development and sporophyte formation. The present study revealed that each and every plant requires different nutrient composition and sucrose for their development. The spore germinationwas inhibited by the sucrose supplementedmedia, basalmedia showed the spore germination. The highest percentage (82.6±0.72) of sporophyte emergence was observed in KC medium supplemented with 1% sucrose (Cheilanthes viridis (Forssk.) Swartz)., (83.8±1.31) of sporeling emergence was observed inMimediumsupplemented with 1%sucrose (Phlebodium aureum L.), KC augmented with 3% sucrose and KN with all the concentrations of sucrose showed sporeling emergence Pronephrium triphyllum (Sw.) Holttum, and KN medium supplemented with 1% sucrose showed high percentage (78.3±1.21) sporeling formation in Sphaerostephanos unitus (L.) (Holttum). The basal medium promotes the gametophyte formation compared to the sporophytes formation. In Sphaerostephanos unitus the apogamous sporophytes formation was stimulated by the sucrose supplementation, 1% sucrose supplemented media inoculated presexual gametophytes induced the apogamous sporophytes formation.