Abstracto

INSULIN resistance affects the expression of the subset of proliferation- related genes in blood cells of obese boys

Dmytro O.Minchenko, Vadym V.Davydov, Olena A.Budreiko, Oksana S. Moliavko, OleksandraV.Tiazhka, OleksandrH.Minchenko

The development of obesity and its metabolic complicationsis associated with dysregulation of numerous intrinsic mechanisms, which control most basic metabolic processes, including insulin sensitivity and cellular proliferation. We studied the expression of genes, which responsible for control of cell proliferation, in bloodcells of obese boys with normal and impaired insulin sensitivity as well asin lean (control) individuals. It was shown that the expression level of ING1, DKK, andSH2B3genes is increased, butEXO1 and CIDEAgenes is decreased in bloodcells of obese boys with normal insulin sensitivity as compared to control group. Insulin resistance in obese boys leads toup-regulation of EXO1 andPER1gene expressions as well as to down-regulation of CIDEA, ING1, DKK, PER2,andSH2B3genesas compared to obese patients with normal insulin sensitivity. Results of this study provide evidence that obesity affects the expression of the subset of proliferation-related genes in bloodcells and that impaired insulin sensitivity in obesity is associated with changes in the expression level of EXO1,PER1, CIDEA, ING1, DKK, PER2,andSH2B3genes, which possibly contribute to the development of obesity, insulin resistance, and glucose intolerance and may reflect, at least in part, some changes in fat tissue.