Abstracto

Production of hyoscyamine and scopolamine using stress inDaturametelL.

S.B.Zimare, N.P.Malpathak

Highest biomass in terms of FreshWeight (FW) and DryWeight (DW) obtained on 2, 4-D (1 mg/l) for all studied medium, and for higher alkaloids production i.e. for hyoscyamine and scopolamine we found that 2, 4-D, BAP (0.5, 0.5mg/l)was the bestmedium.So thismediumwasused formanipulation of alkaloids using different stress producing agents (elicitors) and therefore it was used to carry out further experiment. Highest hyoscyamine and scopolamine contentwas obtained at 0.25mMtreatment of Salicylic acid (SA)which was 0.254mg/gDWand 8.588mg/gDWrespectively, at 36 h. High level of hyoscyamine (0.224mg/g DW) and scopolamine (8.490 mg/g DW) was obtained at 200 mg/l of YE treatment at 36 h. Highest hyoscyamine and scopolamine contentwas obtained at pH5.5 at 12 hand itwas 0.217mg/gDW, 8.333 mg/g DWrespectively.