Abstracto

The study of oxidative response of V79 cells following cyclodextrins treatment

Masoud Mashhadi Akbar Boojar

Cyclodextrins (CDs) are cyclic oligosaccarides with a hydrophobic central cavity, currently used to increase the amount of nonpolar molecules delivery to biological systems. We evaluated cytotoxicity effect of -, - and hydroxypropil--cyclodextrin and oxidative status in V79 cell line with regard to reactive oxygen species generation and macromolecule damages. Cytotoxicitywas assessed byMTT test in the range of doses of 0 to 10 mM. The activity of antioxidant enzymes; superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), togetherwith oxidative damage biomarkers;malondialdehyde (MDA), dityrosine and 8-OH-deoxyguanosine (8-OH-dG) weremeasured by spectrometry andHPLCmethods. The viability of cells was inhibited by these compounds at concentrations of 5 mM and higher and was considerable for - and HP--CD respectively at 10 mM. They promoted ROS generation, increased enzyme activities and elevated the levels ofMDAand dityrosine inwhichHP--CDwasmore effective. Treating cellswith 30 Mof -tocopherol in addition to 10mMofCDs showed significant decrease on the levels of enzyme activities, MDA and dityrosine. As conclusion the present study documents the oxidative radicals forming ability of the studied cyclodextrins and further strengthens the documentation of their cytotoxicity effects through lipid and proteins oxidation damages particularly at levels higher than their ordinary administration levels.