Abstracto

Antioxidant and antiapoptotic effects of Cystoseira myrica on hepatic dysfunction in alloxan-induced Diabetes mellitus in male albino rats

M.A.Nagy

Background: Cystoseira myrica is considered to have protective effects against several diseases. The hepatic dysfunction associated with Diabetes mellitus (DM) has been reported and was found to be associated with oxidative damage. This study was conducted to evaluate the role of Cystoseira myrica to protect against alloxan -induced liver dysfunction in rats .Method:Alloxan was administered i.p. in a single dose (150mg/kg ) to adultmale rats.Alloxan-induced diabetic rats were orally administered hot water extract of Cystoseira myrica (HWCM) 400 mg/kg body weight of rats daily for 30 days after alloxan injection. Result: Alloxan administration to rats resulted in non significant elevation of serum transaminases (sALT and sAST), depletion of hepatic reduced glutathione (GSH), catalase (CAT) and glutathione peroxidase (GPx), elevation of lipid peroxides (LPO) expressed as malondialdehyde (MDA). Significant rises in liver tumor necrosis factor-alpha (TNF-) and caspase-3 levels were noticed in alloxan-induced diabetic. Treatment of the alloxan-induced diabetic rats withHWCM not significantly prevented the elevations of sALT and sAST nor inhibited depletion of hepaticGSH, GPx, CAT andMDAaccumulation. Furthermore, HWCM had normalized hepatic TNF- and caspase-3 levels of alloxaninduced diabetic rats In addition, HWCM prevented the alloxan -induced apoptosis and liver injury as indicated by the liver histopathological analysis. Conclusion: our data indicate that HWCM protects against alloxan - induced liver injury in rats through anti-inflammatory and antiapoptotic mechanisms. However, further merit investigations are needed to verify these results and to assess the efficacy of HWCM therapy to counteract the liver dysfunction and oxidative stress status.