Abstracto

Direct determination of quinine from human excretory product by background reduction and single wavelength detection with reversed-phase H.P.L.C.

Ronald Bartzatt

The pupose of this work is to assay quinine which is used to treat Plasmodiumfalciparum, which is the most common cause of severe and life-threatening malaria. Prompt administration of parenteral or intravenous anti-malarial agents is crucial for patient recovery. The result of this work is a tractable process to monitor compliance of drug administration and levels of quinine drug in vivo will enhance clinical response. Quinine is assayed directly from human urine that is filter sterilized and injected itself onto a reversed-phase C-18 column with no column related problems. The major concluding outcomes include the detection at a single wavelength of 254 nmshowed to be effective and efficaciouslymarginalized all chromatogram peaks associated with human urine. In this work the detection level of quinine was taken as low as 2.25E-05 molar to upper level 4.61E-04 molar. The standard curve produced a high Pearson r correlation of 0.9922 (very high correlation),with a coefficient of determination (R2) at 0.9845. Percent recovery of quinine was 93 % to 115 % with standard deviation of 7.8 %. This highly sensitive methodology for quinine analysis directly from urine will be useful in determining patient compliance and regimenmaintenance.