Abstracto

Effect of Ageing on Permeability Perturbation in Mung Beam (Phasealus aureus Roxb.) Cuttings and its Control by Supplying Parsley Extract I: Membrane Structure and Efflux

Abdullah I. Shaheed and Mohammed A. Jabor

Effect of ageing on permeability perturbation in terms of rooting response of mung beam cuttings has been studied. The shortage of protein and phospholipids levels was considered as indicators of permeability perturbation during ageing phenomenon. Meanwhile percentage of electrolytes leakage in terms of electrical conductivity and average of ions leakage (efflux) such as Ca2+ and Mg2+ in different parts of the cuttings have been measured. In addition, aqueous extract of parsley (Petroselinum crispum Mill.) seeds was used to control meanwhile, the processes that occurs during ageing. The results revealed that ageing caused diminishing of rooting response in aged cuttings (held in d/H2O during ageing period) compared to fresh cuttings. However, a decline in proteins and phospholipids was coincided with permeability perturbation that represented by increment of ions efflux as mentioned above. On the other hand, results of ageing control by holding cuttings in aqueous extract of parsley seeds (1%) instead of d/H2O, revealed a complete stopping (overcome) of the processes that occur during ageing. These processes lead to diminish rooting response of subsequent auxin supply. The control of ageing in terms of membrane repair implies maintaining of protein and phospholipids levels in aged cuttings. Thereafter, prevent ions leakage that occurs due to permeability perturbation.