Abstracto

New analytical methods development for estimation of tianeptine in its tablet form by visible spectrophotometry

Kalyana Ramu Buridi

The aim of the investigation was to see the simple and sensitive visible spectrophotometric methods for the determination of the tianeptine sodium in bulk and tablet dosage forms. Two direct, simple and sensitive visible spectrophotometric methods (M1 and M2) are described for the assay of Tianeptine sodiumin pure and solid dosage forms. The methodM1 involves oxidative coupling of tianeptine with brucine in presence of sodium meta periodate and purple red colored species is formed and exhibits absorption maxima at 510nm. The method M2 is based on the formation of yellowish brown colored species by the drug with Folin reagent and exhibits absorptionmaxima at 450 nm. BeerÂ’s law obeyed in the concentration range of 8-24µg/ml and 16-80 µg/ml for methodM1 and M2 respectively. No interference was observed from the usually existing additives in pharmaceutical formulations and the applicability of the methods was examined by analyzing STABLON tablets containing TIA and the results are statistically compared with those obtained by the UV reference method and validatedwith respect to accuracy, precision, linearity, limit of detection, percentage of recovery, repeatability The reported methods for its assay involve sophisticated equipment, which are very costly and pose problems of maintenance. To overcome these problems, the use of visible spectrophotometric technique is justifiable. The statistical data proved the accuracy, reproducibility and the precision of the proposed methods.