Abstracto

Two simple UV-spectrophotometry - Area under curve technique for quantitative estimation of lamivudine in bulk and in tablets

Atul A.Shirkhedkar, Banty H.Jagnani

Two economical, simple and precise UV-Spectrophotometricmethods have been established for the quantification of Lamivudine (LMV) in tablets. In ‘Method IÂ’, Area under Curve (AUC) was measured in the wavelength range of 255.20–281.40 nm.While, in ‘Method IIÂ’, zero order spectrum of LMV was derivatized into first order (Ä ë = 4) using software UV-probe and AUC was calculated in wavelength range between 273.40 - 296.80 nm. In ‘Method IÂ’ and ‘Method IIÂ’, LMV obeyed linearity in the concentration range of 5 - 30 µg/mL with r2> 0.99. Calibration curves were constructed using instrument response between choosen wavelengths and concentrations of analyte in the solution. The proposed methods were effectively executed out for the qualitative estimation of LMV in marketed tablets and %amounts of LMV estimated by ‘Method IÂ’ and ‘Method IIÂ’ were established to be 99.52%and 100.55%, respectively. The proposed methods were validated for precision, accuracy and ruggedness as per ICH Guidelines.