Abstracto

Wavelength control and spectrophotometric analysis using personal computer

B.Rama Murthy, Ch.V.V.Ramana, N.Anju Latha, U.Eranna, G.Pushpa Chakrapani

Spectrophotometer is an analytical instrument used in research and industry to characterize the materials. In this, the transmission or reflection of the sample ismeasured as a function ofwavelength. The main objective of the paper is to convert the analog single beam UV-Visible manually operated Spectrophotometer into a wavelength control and spectrophotometric analysis (automatic) using personal computer. A software package was also developed for the wavelength selection in the 340-960 nm ranges and for the evaluation of Transmittance/Absorbance/Concentration of any chemical substance. With this modification there was a tremendous reduction in the time required for the qualitative and quantitative analysis. Printing of the absorption spectrumand identification of the absorbance peaks of any given sample was also made possible with this computerized instrument. The performance of this modified instrument was evaluated using the standard annular grade samples. In this modified instrument high resolution grating monochromatic, high sensitive photodiode (for entire spectral range) and high speed wavelength scanning. The results are well comparable with the standard values. The paper deals with hardware and software.